Visualization of caspase-3-like activity in cells using a genetically encoded fluorescent biosensor activated by protein cleavage.

@article{Zhang2013VisualizationOC,
  title={Visualization of caspase-3-like activity in cells using a genetically encoded fluorescent biosensor activated by protein cleavage.},
  author={Jiao Zhang and Xiaodong Wang and Wenjing Cui and Wenwen Wang and Huamei Zhang and Lu Liu and Zicheng Zhang and Zhensheng Li and Guoguang Ying and Ning Zhang and Binghui Li},
  journal={Nature communications},
  year={2013},
  volume={4},
  pages={
          2157
        }
}
Cytosolic caspase-3-like proteases, such as caspase-3 and caspase-7, have a central role in mediating the progress of apoptosis. Here to conveniently monitor caspase-3-like activity in the multicellular environment, we have developed genetically encoded switch-on fluorescence-base indicators that are cyclized chimeras containing a caspase-3 cleavage site as a switch. When cleaved by caspase-3-like proteases, the non-fluorescent indicator rapidly becomes fluorescent, and thus detects in real… CONTINUE READING
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