Visualization of capsule formation by Erwinia amylovora and assays to determine amylovoran synthesis.

@article{Bellemann1994VisualizationOC,
  title={Visualization of capsule formation by Erwinia amylovora and assays to determine amylovoran synthesis.},
  author={Peter Bellemann and Stefan Bereswill and Stefan Berger and Klaus Geider},
  journal={International journal of biological macromolecules},
  year={1994},
  volume={16 6},
  pages={290-6}
}
Exopolysaccharide (EPS) synthesis by Erwinia amylovora depends on environmental and genetic predispositions. To measure the amount of the acidic EPS amylovoran synthesized by E. amylovora cell cultures, a turbidity assay using cetylpyridinium salt was developed. The EPS produced by bacteria grown on solid media was additionally characterized by its water content. The amylovoran capsules were visualized in situ by staining with fluorescein isothiocyanate (FITC)-labelled lectin from Abrus… CONTINUE READING

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