Visualization of axonally transported horseradish peroxidase using enhanced immunocytochemical detection: a direct comparison with the tetramethylbenzidine method.

@article{Romero1999VisualizationOA,
  title={Visualization of axonally transported horseradish peroxidase using enhanced immunocytochemical detection: a direct comparison with the tetramethylbenzidine method.},
  author={Mario I. Romero and Murilo Ara{\'u}jo Romero and George L. Smith},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1999},
  volume={47 2},
  pages={265-72}
}
Visualization of the neuronal tract tracer horseradish peroxidase (HRP) is commonly achieved through the histochemical detection of its enzymatic activity using 3,3',5,5'-tetramethylbenzidine (TMB) as a chromogen. However, the TMB product is unstable and is incompatible with tissue processing methods that render the enzyme inactive, or when a combination of HRP tract tracing with neuronal phenotype identification is required. In this study we evaluated the applicability of the… CONTINUE READING