Visualization of antibody binding to the photosynthetic membrane: the transmembrane orientation of cytochrome b-559.

Abstract

We have used immuno-gold labeling and electron microscopy to study the topography of thylakoid membrane polypeptides. Thylakoid vesicles formed by passage through a French press were adsorbed onto a plastic film supported by an electron microscope grid and processed for single or double immuno-gold labeling. After shadowing with platinum, the inside-out and… (More)

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