Visualization of a filamentous nucleoskeleton with a 23 nm axial repeat.

@article{Jackson1988VisualizationOA,
  title={Visualization of a filamentous nucleoskeleton with a 23 nm axial repeat.},
  author={David A. Jackson and Peter R. Cook},
  journal={The EMBO journal},
  year={1988},
  volume={7 12},
  pages={3667-77}
}
Whether nucleoskeletons seen after extracting cells are preparative artefacts is controversial. Using an extraction method that preserves vital nuclear functions, we have visualized part of a nucleoskeleton by electron microscopy of thick resinless sections. Cells encapsulated in agarose microbeads are lysed using Triton in a physiological buffer; the agarose coat prevents aggregation and protects fragile cell contents. These extracted cells are accessible to small molecules and transcribe and… CONTINUE READING