Visualization of TGN to endosome trafficking through fluorescently labeled MPR and AP-1 in living cells.

@article{Waguri2003VisualizationOT,
  title={Visualization of TGN to endosome trafficking through fluorescently labeled MPR and AP-1 in living cells.},
  author={Satoshi Waguri and Fr{\'e}d{\'e}rique Dewitte and Roland Le Borgne and Yves Rouill{\'e} and Yasuo Uchiyama and J F Dubremetz and Bernard Hoflack},
  journal={Molecular biology of the cell},
  year={2003},
  volume={14 1},
  pages={
          142-55
        }
}
We have stably expressed in HeLa cells a chimeric protein made of the green fluorescent protein (GFP) fused to the transmembrane and cytoplasmic domains of the mannose 6-phosphate/insulin like growth factor II receptor in order to study its dynamics in living cells. At steady state, the bulk of this chimeric protein (GFP-CI-MPR) localizes to the trans-Golgi network (TGN), but significant amounts are also detected in peripheral, tubulo-vesicular structures and early endosomes as well as at the… CONTINUE READING