Visualization of Synaptic Activity in Hippocampal Slices with FM1-43 Enabled by Fluorescence Quenching

@article{Pyle1999VisualizationOS,
  title={Visualization of Synaptic Activity in Hippocampal Slices with FM1-43 Enabled by Fluorescence Quenching},
  author={Jason L. Pyle and Ege T Kavalali and Sukwoo Choi and Richard W. Tsien},
  journal={Neuron},
  year={1999},
  volume={24},
  pages={803-808}
}
Fluorescence imaging of presynaptic uptake and release of styryl dyes such as FM1-43 has provided valuable insights into synaptic function. However, in studies of CNS neurons, the utility of these dyes has been severely limited by nonsynaptic background fluorescence. This has thwarted the use of FM dyes in systems more intact than dissociated neuronal cultures. Here, we describe an approach to selectively reduce undesired fluorescence through quenching of the surface-bound FM1-43 signal. The… CONTINUE READING