Visualization of Chromosomes in Single Human Blastomeres


Preimplantation genetic diagnosis (PGD) makes it possible to select genetically normal embryos for patients at risk of having children with genetic and chromosomal disorders. The major limitation of this technique is the amount of material available for genetic analysis. It usually involves either the first and second polar bodies or only one or two blastomeres biopsied at the cleavage stage (1,2). FISH analysis of the first polar bodies has been used to detect chromosomal translocations in human oocytes (3,4). However, this technique is limited for maternally derived translocations and since, at present, there is no reliable method for visualizing blastomere chromosomes, paternally derived translocations cannot be detected at the preimplantation stage. Nuclear transplantations have been extensively used for the study of nucleocytoplasmic interactions, cell differentiation, and animal and embryo cloning (reviewed in Ref. 5). It is usually expected that heterologous nuclei, if transferred into enucleated eggs and zygotes, would undergo remodeling in accordance with the developmental program of the ooplasm. In this study, we investigated the possibility of applying nuclear transplantation techniques for karyotyping a single blastomere of preimplantation human embryo. Our expectations were based on the fact that individual blastomeres, when fused with ooplasts, will be involved in the cell cycle, characteristic for recipient ooplasm and thus, the timing of mitosis could be manipulated and predicted. MATERIALS AND METHODS

DOI: 10.1023/A:1022579731014

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@article{Evsikov1999VisualizationOC, title={Visualization of Chromosomes in Single Human Blastomeres}, author={Sergei Evsikov and Yury Verlinsky}, journal={Journal of Assisted Reproduction and Genetics}, year={1999}, volume={16}, pages={133-137} }