Visualization and enumeration of marine planktonic archaea and bacteria by using polyribonucleotide probes and fluorescent in situ hybridization.

@article{DeLong1999VisualizationAE,
  title={Visualization and enumeration of marine planktonic archaea and bacteria by using polyribonucleotide probes and fluorescent in situ hybridization.},
  author={Edward F DeLong and Lance Trent Taylor and Terence Marsh and Christina M. Preston},
  journal={Applied and environmental microbiology},
  year={1999},
  volume={65 12},
  pages={5554-63}
}
Fluorescent in situ hybridization (FISH) using rRNA-specific oligonucleotide probes has emerged as a popular technique for identifying individual microbial cells. In natural samples, however, the signal derived from fluor-labeled oligonucleotide probes often is undetectable above background fluorescence in many cells. To circumvent this difficulty, we applied fluorochrome-labeled polyribonucleotide probes to identify and enumerate marine planktonic archaea and bacteria. The approach greatly… CONTINUE READING

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The ecology of Paraphysomonas imperforata based on studies employing oligonucleotide probe identification in coastal water samples and enrichment cultures

E. L. Lim, M. R. Dennett, R. Mark, D. A. Caron
Limnol. Oceanogr • 1999

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