Visual detection of DNA from salmonella and mycobacterium using split DNAzymes.

@article{Darius2010VisualDO,
  title={Visual detection of DNA from salmonella and mycobacterium using split DNAzymes.},
  author={Aw Kang Lie Darius and Neo Jia Ling and Uttamchandani Mahesh},
  journal={Molecular bioSystems},
  year={2010},
  volume={6 5},
  pages={
          792-4
        }
}
  • Aw Kang Lie Darius, Neo Jia Ling, Uttamchandani Mahesh
  • Published 2010
  • Medicine, Biology
  • Molecular bioSystems
  • The split G-quadruplex DNAzyme has emerged as a valuable tool for visual DNA detection. Most reports on its use have however been constrained to proof-of-concept demonstrations using synthetic oligonucleotides. This is due to the inherent complexities of the assay, and the multiple components involved. Herein, we have overcome several of these challenges and have successfully integrated asymmetric PCR with the visual detection step, allowing enriched targets from complex samples to be… CONTINUE READING

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