Visual and sensitive detection of viable pathogenic bacteria by sensing of RNA markers in gold nanoparticles based paper platform.

@article{Liu2014VisualAS,
  title={Visual and sensitive detection of viable pathogenic bacteria by sensing of RNA markers in gold nanoparticles based paper platform.},
  author={Hongxing Liu and Fangfang Zhan and Fang Liu and Minjun Zhu and Xiaoming Zhou and Da Xing},
  journal={Biosensors & bioelectronics},
  year={2014},
  volume={62},
  pages={
          38-46
        }
}
Food-borne pathogens have been recognized as a major cause of human infections worldwide. Their identification needs to be simpler, cheaper and more reliable than the traditional methods. Here, we constructed a low-cost paper platform for viable pathogenic bacteria detection with the naked eye. In this study, an effective isothermal amplification method was used to amplify the hlyA mRNA gene, a specific RNA marker in Listeria monocytogenes. The amplification products were applied to the paper… CONTINUE READING
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