In this paper, we propose a visual feedback system for a neuroanatomy. The stereoscopic microscope image of the dentate gyrus of adult rat brain in the neuroanatomy is converted into a black and white image. By the region segmentation, the region of the dentate gyrus is extracted. A binary image is obtained from a microscopic photo of cresyl violet-stained coronal section of the rat brain as a template. By the template matching, the apex of the dentate gyrus is automatically detected. The visual feedback system consists of a stereoscopic microscope, a micromanipulator, two CCD cameras and a personal computer. According to the stereo vision method, the distance between the dentate gyrus and the tip of microelectrode is three dimensionally measured based on the position difference between the left and the right image. Following an insertion of the tip of microelectrode to the dentate gyrus, the lypophilic fluorescent tracer is slowly injected by a nanoinjector. As a result, the glass microelectrode can be guided to pierces the apex of the dentate gyrus in desired depth.