Viscosity-dependent structural fluctuations in enzyme catalysis.

  title={Viscosity-dependent structural fluctuations in enzyme catalysis.},
  author={Benjamin Gavish and M. M. Werber},
  volume={18 7},
The effect of viscosity on the rate of catalysis of carboxypeptidase A has been tested. By use of the tripeptide carbobenzoxy-l-alanyl-l-alanyl-l-alanine [Z(L-Ala)3] as substrate, it was shown that most of the effect on the hydrolysis rate caused by the presence of 30 or 40% methanol or glycerol in aqueous solution can be ascribed to a contribution of viscosity to the catalytic rate constant, kcat. Arrhenius plots of kcat in 30 and 40% glycerol or methanol are linear and almost parallel. When… CONTINUE READING

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