Virus isolation from wild-caught mosquitoes during a Japanese encephalitis outbreak in Kerala in 1996.

Abstract

Out of 5357 wild-caught mosquitoes in 163 pools tested for virus using antigen capture ELISA and an insect-bioassay (inoculation into Toxorhynchites splendens larvae and identification by IFA using JE virus-specific monoclonal antibody), 16 flavivirus isolations were made of which 12 (75%) were identified as JE virus. Of the 12 JE virus isolations, 7 were from Culex tritaeniorhynchus, 3 from Mansonia uniformis and 1 each from Ma. indiana and Anopheles subpictus. Four isolations from Mansonia species for the first time reported here are noteworthy.

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@article{Dhanda1997VirusIF, title={Virus isolation from wild-caught mosquitoes during a Japanese encephalitis outbreak in Kerala in 1996.}, author={V. Dhanda and Velayutham Thenmozhi and N. Pradeep Kumar and Joghee Gowder Hiriyan and Natarajan Arunachalam and Arumugham Balasubramanian and A Ilango and A Gajanana}, journal={The Indian journal of medical research}, year={1997}, volume={106}, pages={4-6} }