Virus-induced gene silencing (VIGS) as a reverse genetic tool to study development of symbiotic root nodules.

@article{Constantin2008VirusinducedGS,
  title={Virus-induced gene silencing (VIGS) as a reverse genetic tool to study development of symbiotic root nodules.},
  author={Gabriela D. Constantin and Mette Gr\onlund and Ida Elisabeth Johansen and Jens Stougaard and Ole S\ogaard Lund},
  journal={Molecular plant-microbe interactions : MPMI},
  year={2008},
  volume={21 6},
  pages={720-7}
}
Virus-induced gene silencing (VIGS) can provide a shortcut to plants with altered expression of specific genes. Here, we report that VIGS of the Nodule inception gene (Nin) can alter the nodulation phenotype and Nin gene expression in Pisum sativum. PsNin was chosen as target because of the distinct non-nodulating phenotype of nin mutants in P. sativum, Lotus japonicus, and Medicago truncatula. The vector based on Pea early browning virus (PEBV) was engineered to carry one of three… CONTINUE READING