Virus Induced Gene Silencing of a DEFICIENS Ortholog in Nicotiana Benthamiana

@article{Liu2004VirusIG,
  title={Virus Induced Gene Silencing of a DEFICIENS Ortholog in Nicotiana Benthamiana},
  author={Yule Liu and Naomi Nakayama and Michael M Schiff and Amy Litt and Vivian F. Irish and Savithramma P Dinesh-Kumar},
  journal={Plant Molecular Biology},
  year={2004},
  volume={54},
  pages={701-711}
}
Traditionally, developmental studies in plant biology have suffered from the lack of a convenient means to study gene function in non-model plant species. Here we show that virus-induced gene silencing (VIGS) is an effective new tool to study the function of orthologs of floral homeotic genes such as DEFICIENS(DEF) in non-model systems. We used a tobacco rattle virus (TRV)-based VIGS approach to study the function of the Nicotiana benthamiana DEFortholog (NbDEF). Silencing of NbDEFin N… CONTINUE READING