Virulence genotyping of Salmonella spp. with multiplex PCR.

@article{Skyberg2006VirulenceGO,
  title={Virulence genotyping of Salmonella spp. with multiplex PCR.},
  author={Jerod A. Skyberg and Catherine Mary Logue and Lisa Kay Nolan},
  journal={Avian diseases},
  year={2006},
  volume={50 1},
  pages={77-81}
}
The purpose of this study was to develop a multiplex polymerase chain reaction (PCR) protocol useful in the virulence genotyping of Salmonella spp. with the idea that genotyping could augment current Salmonella characterization and typing methods. Seventeen genes associated with Salmonella invasion, fimbrial production, toxin production, iron transport, and intramacrophage survival were targeted by three PCR reactions. Most of these genes are required for full Salmonella virulence in a murine… CONTINUE READING