Virulence, serotype and phylogenetic groups of diarrhoeagenic Escherichia coli isolated during digestive infections in Abidjan, Côte d’Ivoire

  title={Virulence, serotype and phylogenetic groups of diarrhoeagenic Escherichia coli isolated during digestive infections in Abidjan, C{\^o}te d’Ivoire},
  author={A. Dadi{\'e} and N. Kouassi and {\'E}. Dako and M. Dje and M. Dosso},
  journal={African Journal of Biotechnology},
The virulence, serotype and phylogenetic traits of diarrhoeagenic Escherichia coli were detected in 502 strains isolated during digestive infections. Molecular detection of the target virulence genes, rfb gene of operon O and phylogenetic grouping genes Chua, yjaA and TSPE4.C2 was performed. Prevalence of strains harbouring virulent genes was 7.8%. The virulent genes eaeA, bfp, stx2, st1, lt, aggA, east1, ipaH, ial, cnf1 and afa were detected. EAEC (36%) and both EPEC and ATEC (25.6%) are the… Expand

Figures and Tables from this paper

Diverse Escherichia coli pathovars of phylogroups B2 and D isolated from animals in Tunisia.
Higher diversity of pathovars which carried diverse combinations of virulence genes in healthy isolates is found, and it seems that the infections were caused by different mechanisms. Expand
Phylogenetic Groups of Escherichia coli Strains from Patients with Urinary Tract Infection in Iran Based on the New Clermont Phylotyping Method
The findings showed the high prevalence of MDR E. coli isolates with dominance of group B2, and such studies need to be done also in other regions to provide greater understanding of the antibiotic resistance pattern and the prevalences of different phylogenetic groups. Expand
Determination of the frequency of β-lactamase genes (bla SHV, bla TEM, bla CTX-M) and phylogenetic groups among ESBL-producing uropathogenic Escherichia coli isolated from outpatients
To determine the antimicrobial resistance pattern, investigate ESBL phenotypes and genotypes and determine the phylogenetic groups among ESBL-positive isolates from outpatients, the presence of CTX-M and resistance to quinolones were more frequent in B2 strains than in non-B2 strains. Expand
Effect of thermals constraints on enteropathogenic Escherichia coli survival in porridges based on maize (Zea mays) or millet (Pennicetum glaucum) flour traditionally made in Côte d’Ivoire
*Corresponding Author E-mail: Tel. +225 07 32 07 36 Enteropathogenic Escherichia coli species are considered as one of the main causes of bacterial gastroenteritis in children. TheExpand
Prevalence of Enteropathogenic Escherichia coli in Maize (Zea mays) or Millet (Pennicetum glaucum) Flours and Porridges
This work aims to demonstrate the efforts towards in-situ applicability of EMMARM, which aims to provide real-time information on food spoilage and spoilage-causing organisms and their phytochemical properties. Expand


[Isolation of enteric pathogeic agents in Côte d'Ivoire: Escherichia coli O157:H7 and enteroaggregative E. coli].
This is the first report of Shiga-toxin producing E. coli (STEC) in Côte d'Ivoire, and given its low prevalence (0.8%), E. Coli does not appear to be a public health problem in the environment. Expand
Molecular epidemiology of Escherichia coli isolated from young South African children with diarrhoeal diseases.
PCR and tissue culture assays were shown to be useful techniques for the epidemiological study of E. coli where this organism is a major cause of diarrhoea. Expand
Characterisation of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: a microbiological study.
Augmented adherence of the strain to intestinal epithelium might facilitate systemic absorption of Shiga toxin and could explain the high progression to haemolytic uraemic syndrome. Expand
Virulence factors of uropathogenic Escherichia coli from a university hospital in Ribeirão Preto, São Paulo, Brazil.
The strains isolated from the outpatients displayed a greater number of virulence factors compared to those from hospitalized subjects, emphasizing the difference between these two kinds of patients. Expand
Enterohemorrhagic Escherichia coli
The severity of the illness it causes combined with its apparent low infectious dose qualifies E. coli O157:H7 to be among the most serious of known foodborne pathogens. Expand
No evidence of the Shiga toxin-producing E. coli O104:H4 outbreak strain or enteroaggregative E. coli (EAEC) found in cattle faeces in northern Germany, the hotspot of the 2011 HUS outbreak area
The data support the theory, that, in contrast to other Shiga-toxin producing E. coli, cattle are not the reservoir for the O104:H4 outbreak strain or other EAEC, but that the outbreak strain seems to be adapted to humans or might have yet another reservoir, raising new questions about the epidemiology of STEC O104;H4. Expand
Identification of Escherichia coli O-serogroups by restriction of the amplified O-antigen gene cluster (rfb-RFLP).
0-serogroups of clinical isolates were deduced accurately from O-patterns in all cases, even for some rough or nonagglutinating isolates, and the restriction method (rfb-RFLP) may prove to be better than serotyping since 100% of strains are typable, which is not the case with serotypes. Expand
Virulence properties of Escherichia coli O111:H12 strains.
It is concluded that the O111:H12 serotype, one of the first E. coli identified in infantile diarrhea, belongs to the enteroaggregative E. Escherichia coli category but the genes encoding its adherence phenotype are distinct from those previously described. Expand
Organization of Escherichia coli O157 O Antigen Gene Cluster and Identification of Its Specific Genes
  • Lei Wang, P. Reeves
  • Biology, Medicine
  • Infection and Immunity
  • 1998
The method for fast sequencing of O antigen gene clusters plus an ability to predict which genes will be O antigen specific will enable PCR tests to be developed as needed for other clones of E. coli or, once flanking genes are identified, clones of any gram-negative bacterium. Expand
Pathogenic enteric Escherichia coli in children with and without diarrhea in Maputo, Mozambique.
To the best of the authors' knowledge, this is the first report in the literature on the circulation of potentially diarrheagenic E. coli in Mozambique. Expand