Virtual metagenome reconstruction from 16S rRNA gene sequences.

  title={Virtual metagenome reconstruction from 16S rRNA gene sequences.},
  author={Shujiro Okuda and Yuki Tsuchiya and Chiho Kiriyama and Masumi Itoh and Hisao Morisaki},
  journal={Nature communications},
Microbial ecologists have investigated roles of species richness and diversity in a wide variety of ecosystems. Recently, metagenomics have been developed to measure functions in ecosystems, but this approach is cost-intensive. Here we describe a novel method for the rapid and efficient reconstruction of a virtual metagenome in environmental microbial communities without using large-scale genomic sequencing. We demonstrate this approach using 16S rRNA gene sequences obtained from denaturing… CONTINUE READING
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Phylogenetic analysis of a spontaneous cocoa bean fermentation metagenome reveals new insights into its bacterial and fungal community diversity

  • K. Illeghems, L. De Vuyst, Z. Papalexandratou, S. Weckx
  • PLoS ONE 7,
  • 2012

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