Virtual Terminator nucleotides for next generation DNA sequencing

Abstract

We synthesized reversible terminators with tethered inhibitors for next-generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides, and we used them to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high-quality sequence data without the need for target amplification and thus avoided concomitant biases.

DOI: 10.1038/nmeth.1354

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@inproceedings{Bowers2009VirtualTN, title={Virtual Terminator nucleotides for next generation DNA sequencing}, author={Jayson L Bowers and Judith S Mitchell and Eric Beer and Philip R. Buzby and Marie Causey and J. William Efcavitch and M. E. Jarosz and Edyta Krzymanska-Olejnik and Li A. Kung and Doron Lipson and Geoffrey M. Lowman and Subramanian Marappan and Peter McInerney and Adam R Platt and Atanu Roy and Suhaib M. Siddiqi and Kathleen E Steinmann and John Thompson}, booktitle={Nature methods}, year={2009} }