Virtual 2-D gel electrophoresis: visualization and analysis of the E. coli proteome by mass spectrometry.

@article{Loo2001Virtual2G,
  title={Virtual 2-D gel electrophoresis: visualization and analysis of the E. coli proteome by mass spectrometry.},
  author={Rachel R Ogorzalek Loo and James D. Cavalcoli and Ruth A Vanbogelen and Cecily Mitchell and Joseph A Loo and Brian Moldover and Philip C. Andrews},
  journal={Analytical chemistry},
  year={2001},
  volume={73 17},
  pages={4063-70}
}
Mass spectrometric surface analysis of isoelectric focusing gels provides an ultrasensitive approach to proteome analysis. This "virtual 2-D gel" approach, in which mass spectrometry is substituted for the size-based separation of SDS-PAGE, provides advantages in mass resolution and accuracy over classical 2-D gels and can be readily automated. Protein identities can be postulated from molecular mass (+/-0.1-0.2% for proteins of <50 kDa in size) and pI (+/-0.3 pH unit) and confirmed by MALDI in… CONTINUE READING