Viral proteomics: global evaluation of viruses and their interaction with the host

@article{Viswanathan2007ViralPG,
  title={Viral proteomics: global evaluation of viruses and their interaction with the host},
  author={Kasinath Viswanathan and Klaus Fr{\"u}h},
  journal={Expert Review of Proteomics},
  year={2007},
  volume={4},
  pages={815 - 829}
}
Viruses constantly adapt to and modulate the host environment during replication and propagation. Both DNA and RNA viruses encode multifunctional proteins that interact with and modify host cell proteins. While viral genomes were the first complete sequences known, the corresponding proteomes are only now elucidated, with some surprising results. Even more daunting is the task to globally monitor the impact of viral infection on the proteome of the host cell and many technical hurdles must… Expand
Protein arrays as tool for studies at the host-pathogen interface.
TLDR
This review focuses on techniques analyzing the state-of-the-art of proteomic technologies and their possibilities to diagnose and explore host-pathogen interactions, and major technical advancements, applications and protocol concerns are presented. Expand
Functional Proteomics Screening for Novel Anti-viral Drug Targets
TLDR
Functional proteomics screening platforms have globally characterized host–virus interactions and host functions important for viral infection and thus facilitated discovery of either novel or existing licensed drugs with anti-viral activity. Expand
Proteomic analysis of purified Newcastle disease virus particles
TLDR
The current study presented the first standard proteomic profile of NDV, demonstrating the incorporation of cellular proteins in NDV particles, which provides valuable information for elucidating viral infection and pathogenesis. Expand
Deciphering Novel Host–Herpesvirus Interactions by Virion Proteomics
  • R. Lippé
  • Biology, Medicine
  • Front. Microbio.
  • 2012
TLDR
A mini review of cellular proteins detected in herpesviruses highlights the common cellular functions of these proteins, their potential implications for host–pathogen interactions, discusses technical limitations, the need for complementing methods and probes potential future research avenues. Expand
Profiling of cellular proteins in porcine reproductive and respiratory syndrome virus virions by proteomics analysis
TLDR
The present study has demonstrated the incorporation of cellular proteins in PRRSV virions, which provides valuable information for the further investigations for the effects of individual cellular proteins on the viral replication, assembly, and pathogenesis. Expand
Differential proteome analysis of host cells infected with porcine circovirus type 2.
TLDR
Colocalization and coimmunoprecipitation analyses confirmed that the cellular alpha tubulin interacts with the Cap protein of PCV2 in the infected PK-15 cells, which brings us a step closer to defining the cellular requirements for the underlying mechanism ofPCV2 replication and pathogenesis. Expand
Bridging the past and the future of virology: Surface plasmon resonance as a powerful tool to investigate virus/host interactions
TLDR
Surface plasmon resonance emerges as an ideal link between conventional biological experimentation and system biology studies functional to the identification of highly connected viral or host proteins that act as nodal points in virus life cycle and thus considerable as therapeutical targets for the development of innovative antiviral strategies. Expand
Innate immune modulation by RNA viruses: emerging insights from functional genomics
TLDR
Systems-biology approaches are increasingly being used to study the processes of viral triggering and regulation of host immune responses and provide new insights into how RNA viruses cause disease. Expand
Proteomics and Host—Pathogen Interactions
TLDR
New ways based on evolutionary concepts are suggested to enable further elucidation of the molecular complexities of host-pathogen genome interactions, to increase the knowledge about the molecular war involved in host–pathogen associations, taking into account the environmental factors. Expand
Connecting viral with cellular interactomes
TLDR
An overview of current approaches and their technical limitations, present recent examples and novel developments, and presents recent examples of novel developments are presented. Expand
...
1
2
3
4
...

References

SHOWING 1-10 OF 86 REFERENCES
Mass spectrometry reveals specific and global molecular transformations during viral infection.
Mass spectrometry analysis was used to target three different aspects of the viral infection process: the expression kinetics of viral proteins, changes in the expression levels of cellular proteins,Expand
New insights into viral structure and virus–cell interactions through proteomics
TLDR
The use of several proteomic approaches for the analysis of HIV–cellular receptor interactions, the molecular mechanisms of transport of herpessimplex virus within neurons, and the structure of the tegument of herpes simplex virus are described. Expand
Identification of cellular proteins modified in response to African swine fever virus infection by proteomics
TLDR
Proteomic technology is used to globally examine African swine fever virus (ASFV)‐infected Vero cells searching for infection‐associated proteins in order to determine target proteins for pathogenesis studies, and constitutes the first attempt to understand the dynamics of ASFV‐host cell interactions by proteomics. Expand
Tracking and Elucidating Alphavirus-Host Protein Interactions*
TLDR
The results demonstrated that G3BP is a nuclear transport factor, as hypothesized previously, and that viral infection may alter RNA transport, and the utility of using a standard GFP tag to both track viral protein localization and elucidate specific viral-host interactions over time in infected mammalian cells. Expand
Vaccinia Virus Proteome: Identification of Proteins in Vaccinia Virus Intracellular Mature Virion Particles
TLDR
This study provides the first comprehensive structural analysis of the infectious vaccinia virus IMV, showing that it contains 75 viral proteins, including structural proteins, enzymes, transcription factors, and predicted viral proteins not known to be expressed or present in the IMV. Expand
Plunder and Stowaways: Incorporation of Cellular Proteins by Enveloped Viruses
TLDR
The roles, or potential roles, that the major cellular proteins found associated with enveloped viruses may play in their life cycles are described and discussed. Expand
Probing viruses with mass spectrometry.
  • G. Siuzdak
  • Chemistry, Medicine
  • Journal of mass spectrometry : JMS
  • 1998
TLDR
Using mass spectrometry, the identification of viral protein post-translational modifications such as myristoylation, phosphorylation and disulfide bridging is described, demonstrating that viruses are highly dynamic particles whose viral capsid's mobility could, until recently, be inferred only from inherently static spectroscopic methods. Expand
Modification of Host Lipid Raft Proteome upon Hepatitis C Virus Replication*S
TLDR
It is indicated that protein subcellular relocalization occurs in HCV-containing cells that can directly affect HCV replication and small interfering RNAs directed to the modulated small GTPases Cdc42 and RhoA resulted in decreased replication of HCV. Expand
Genome-wide analysis of vaccinia virus protein-protein interactions.
TLDR
In the majority of cases, one of the interacting proteins was known to be involved in DNA replication, transcription, virion structure, or host evasion, thereby providing a clue to the role of the other uncharacterized protein in a specific process. Expand
Identification of cellular interaction partners of the influenza virus ribonucleoprotein complex and polymerase complex using proteomic-based approaches.
TLDR
Proteomics-based approaches represent powerful tools to identify novel vRNP-associated cellular factors for further characterization and revealed that NPM is recruited to sites of viral transcription and replication in infected cells, indicating its role in viral RNA synthesis. Expand
...
1
2
3
4
5
...