Viral proteomics: global evaluation of viruses and their interaction with the host

  title={Viral proteomics: global evaluation of viruses and their interaction with the host},
  author={Kasinath Viswanathan and Klaus Fr{\"u}h},
  journal={Expert Review of Proteomics},
  pages={815 - 829}
Viruses constantly adapt to and modulate the host environment during replication and propagation. Both DNA and RNA viruses encode multifunctional proteins that interact with and modify host cell proteins. While viral genomes were the first complete sequences known, the corresponding proteomes are only now elucidated, with some surprising results. Even more daunting is the task to globally monitor the impact of viral infection on the proteome of the host cell and many technical hurdles must… 

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    Journal of mass spectrometry : JMS
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