Viral hijacking of the TENT4–ZCCHC14 complex protects viral RNAs via mixed tailing

@article{Kim2020ViralHO,
  title={Viral hijacking of the TENT4–ZCCHC14 complex protects viral RNAs via mixed tailing},
  author={Dongwan Kim and Young-suk Lee and Soo-Jin Jung and Jinah Yeo and Jenny J. Seo and Young-Yoon Lee and Jaechul Lim and Hyeshik Chang and Jaewon Song and Jihye Yang and Jong-Seo Kim and G. Jung and Kwangseok Ahn and V. Kim},
  journal={Nature Structural \& Molecular Biology},
  year={2020},
  volume={27},
  pages={581-588}
}
TENT4 enzymes generate ‘mixed tails’ of diverse nucleotides at 3′ ends of RNAs via nontemplated nucleotide addition to protect messenger RNAs from deadenylation. Here we discover extensive mixed tailing in transcripts of hepatitis B virus (HBV) and human cytomegalovirus (HCMV), generated via a similar mechanism exploiting the TENT4–ZCCHC14 complex. TAIL-seq on HBV and HCMV RNAs revealed that TENT4A and TENT4B are responsible for mixed tailing and protection of viral poly(A) tails. We find that… Expand
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