Vinculin: a novel marker for quiescent and activated hepatic stellate cells in human and rat livers

@article{Kawai2003VinculinAN,
  title={Vinculin: a novel marker for quiescent and activated hepatic stellate cells in human and rat livers},
  author={S. Kawai and H. Enzan and Y. Hayashi and Yu Jin and Limei Guo and E. Miyazaki and M. Toi and N. Kuroda and M. Hiroi and T. Saibara and H. Nakayama},
  journal={Virchows Archiv},
  year={2003},
  volume={443},
  pages={78-86}
}
In liver injuries, the quiescent hepatic stellate cells (HSCs) promptly change to activated HSCs, which are easily identified by the intense immunoreactivity for α-smooth muscle actin. However, reproducible markers for quiescent HSCs in formalin-fixed, paraffin-embedded liver tissue sections have not yet been reported. We immunohistochemically examined the expression of vinculin, one major protein of dense plaques, on cultured LI90 cells and on HSCs in normal and diseased human and rat livers… Expand
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The Normal Liver

References

SHOWING 1-10 OF 51 REFERENCES
Immunohistochemical identification of Ito cells and their myofibroblastic transformation in adult human liver
TLDR
Results indicate that immunohistochemistry using an anti-ASMA antibody is a sensitive and reliable method for the identification of both normal and transformed Ito cells in adult human livers. Expand
Class VI intermediate filament protein nestin is induced during activation of rat hepatic stellate cells
TLDR
It is shown that activated stellate cells express nestin, a class VI intermediate filament protein originally identified as a marker for neural stem cells, and that culture activation is a stronger stimulus than in vivo activation by injection of CCl4. Expand
Comparison of glial fibrillary acidic protein and desmin staining in normal and CCl4‐induced fibrotic rat livers
TLDR
GFAP is expressed by a subpopulation of fat‐storing cells, which differs partially from the population that expresses desmin, which means that in chronically injured liver, GFAP may not be as useful as in normal rat liver. Expand
Modulation of alpha smooth muscle actin and desmin expression in perisinusoidal cells of normal and diseased human livers.
TLDR
The results are compatible with the assumption that the appearance of alpha-SM actin and desmin-expressing myofibroblasts results at least in part from a phenotypic modulation of PSCs. Expand
Intrahepatic expression of the hepatic stellate cell marker fibroblast activation protein correlates with the degree of fibrosis in hepatitis C virus infection.
TLDR
FAP expression identifies a HSC subpopulation at the tissue-remodelling interface that is related to the severity of liver fibrosis, as well as a correlation between FAP expression and necroinflammatory score. Expand
Expression of cellular prion protein in activated hepatic stellate cells.
TLDR
Results indicate that PrP expression is closely related to stellate cell activation associated with fibrogenic stimuli. Expand
Synaptophysin: A novel marker for human and rat hepatic stellate cells.
TLDR
It is concluded that synaptophysin is a novel marker for quiescent as well as activated hepatic stellate cells, and this finding raises questions about its embryonic origin and its differentiation. Expand
Hepatic stellate cells express the low affinity nerve growth factor receptor p75 and undergo apoptosis in response to nerve growth factor stimulation.
We have examined the expression of p75, a member of the TNF receptor superfamily in hepatic stellate cells (HSC) and pancreatic stellate cells (PSC). Activated HSC and PSC were demonstrated byExpand
Desmin‐containing stellate cells in rat liver; distribution in normal animals and response to experimental acute liver injury
TLDR
It is confirmed that the perisinusoidal cells in rat liver express the intermediate filament protein, desmin, and this marker is used for identification of the cells on light microscopy and anti‐desmin antibodies are of potential value in further characterizing the functional role of perisinian cells in normal and diseased liver. Expand
Short Communication Expression of Cellular Prion Protein in Activated Hepatic Stellate Cells
Suppression subtractive hybridization was used to clone genes associated with the activation of hepatic stellate cells and 13 genes were found to be dominantly expressed in activated stellate cells.Expand
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