Video-rate nanoscopy enabled by sCMOS camera-specific single-molecule localization algorithms

  title={Video-rate nanoscopy enabled by sCMOS camera-specific single-molecule localization algorithms},
  author={Fang Huang and Tobias M. P. Hartwich and Felix E. Rivera-Molina and Yu Lin and Whitney C. Duim and Jane J. Long and Pradeep Devappa Uchil and Jordan R. Myers and Michelle A. Baird and Walther Mothes and Michael W. Davidson and Derek Toomre and Joerg Bewersdorf},
  booktitle={Nature Methods},
Newly developed scientific complementary metal-oxide semiconductor (sCMOS) cameras have the potential to dramatically accelerate data acquisition, enlarge the field of view and increase the effective quantum efficiency in single-molecule switching nanoscopy. However, sCMOS-intrinsic pixel-dependent readout noise substantially lowers the localization precision and introduces localization artifacts. We present algorithms that overcome these limitations and that provide unbiased, precise… CONTINUE READING
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