Video imaging of walking myosin V by high-speed atomic force microscopy

  title={Video imaging of walking myosin V by high-speed atomic force microscopy},
  author={Noriyuki Kodera and Daisuke Yamamoto and Ryoki Ishikawa and Toshio Ando},
The dynamic behaviour of myosin V molecules translocating along actin filaments has been mainly studied by optical microscopy. The processive hand-over-hand movement coupled with hydrolysis of adenosine triphosphate was thereby demonstrated. However, the protein molecules themselves are invisible in the observations and have therefore been visualized by electron microscopy in the stationary states. The concomitant assessment of structure and dynamics has been unfeasible, a situation prevailing… CONTINUE READING
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