Video-based image collection for quantitative histopathology.


Obtaining histologic images for computer-based morphometric analysis is associated with a number of standardization problems, which must be solved if reproducible data collection is expected. These problems include tissue processing, sectioning and staining, standardizing and calibrating the video camera and determining the appropriate sampling rate (pixels/micron). Suggested solutions for these problems are presented for a specific image analysis system, but are applicable to other systems with similar capabilities. Biologic variability is not eliminated by computer-assisted analysis, so it is important to minimize data-collection artifacts by parallel processing of experimental and control material, as in other investigative work.

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@article{Jordan1988VideobasedIC, title={Video-based image collection for quantitative histopathology.}, author={Scott W. Jordan and James Brayer and Peter H. Bartels and Robert E. Anderson}, journal={Analytical and quantitative cytology and histology}, year={1988}, volume={10 1}, pages={37-46} }