Viable human buccal mucosa cells do not yield typical nucleoids: impacts on the single-cell gel electrophoresis/Comet assay.

@article{Pinhal2006ViableHB,
  title={Viable human buccal mucosa cells do not yield typical nucleoids: impacts on the single-cell gel electrophoresis/Comet assay.},
  author={Danillo Pinhal and Alisson M Gontijo and Victor Alexis Valenzuela Reyes and Daisy Maria F{\'a}vero Salvadori},
  journal={Environmental and molecular mutagenesis},
  year={2006},
  volume={47 2},
  pages={117-26}
}
Buccal mucosa (BM) cells have been used in human biomonitoring studies for detecting DNA adducts and chromosomal damage in an epithelial cell population. In the present study, we have investigated if human BM cells are suitable for use in the single-cell gel electrophoresis (SCGE)/Comet assay as an approach for estimating the exposure of epithelial cells to DNA-damaging agents. Our results indicate that only a few cells from BM cell samples yield comets that can be analyzed by current methods… CONTINUE READING

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