Viable cells survive in fresh frozen human bone allografts.

  title={Viable cells survive in fresh frozen human bone allografts.},
  author={D. Simpson and Gopikrishna K. Kakarala and Karen Hampson and Niall Steele and Brian Ashton},
  journal={Acta orthopaedica},
  volume={78 1},
BACKGROUND Fresh frozen bone allograft is available for human recipients after at least 6 months of quarantine at -80 degrees C. It is assumed that cryopreservation without cryoprotectant removes all viable donor cells. METHODS We studied the in vitro cell growth from samples of fresh frozen human femoral head allografts after they had been released for patient use, and compared it with cell growth from a control group of fresh cancellous bone specimens from excised femoral heads (8 samples… CONTINUE READING
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