In recent years, more and more higher education institutions have interests of integrating internetbased technologies in the classroom as part of the learning environment. Compared to studies on other information technologies, users’ behavior towards this type of systems, however, has not been assessed and understood thoroughly. In order to get more experience about human behaviors on multimedia learning environment, we conducted a comparative study consisting of 362 students, which is almost three times the sample size of the previous study, participating to test the theoretical model. Results suggest that TAM is a solid theoretical model where its validity can extend to the multimedia and e-learning context. The study provides a more intensive view of the multimedia learning system users and is an important step towards a better understanding of the user behavior on the system and a multimedia acceptance model.