Viability of equine articular chondrocytes in alginate beads exposed to different oxygen tensions.

@article{Schneider2004ViabilityOE,
  title={Viability of equine articular chondrocytes in alginate beads exposed to different oxygen tensions.},
  author={Nicole Schneider and J-P Lejeune and Carol M T Deby and Ginette P. Deby-Dupont and Didier D A Serteyn},
  journal={Veterinary journal},
  year={2004},
  volume={168 2},
  pages={167-73}
}
Ischaemia and reperfusion are suspected to alter chondrocyte metabolism. Here, we studied the effects of three oxygen (O2) tensions on the viability of equine articular chondrocytes isolated from the cartilage of the distal interphalangeal joint of horses. Chondrocytes were cultured in alginate beads under 1%, 5% or 21% gas phase O2 concentration for 14 days, cellular growth kinetics were measured (n=6), and the cells were observed by light microscopy after staining for necrotic and apoptotic… CONTINUE READING