Viability assessment of turkey sperm using fluorescent staining and flow cytometry.

@article{Donoghue1995ViabilityAO,
  title={Viability assessment of turkey sperm using fluorescent staining and flow cytometry.},
  author={Ann M. Donoghue and Duane L. Garner and Dan J. Donoghue and Lawrence A. Johnson},
  journal={Poultry science},
  year={1995},
  volume={74 7},
  pages={1191-200}
}
Turkey sperm viability was evaluated using several fluorescent stains both singularly and in combination. Dilution curves and several extenders were used to determine optimal stain concentrations. Semen was collected from eight toms, pooled, diluted, stained, and evaluated microscopically within 2 h of collection. Replicates were assessed for both viable and nonviable sperm (green and red fluorescence, respectively) using flow cytometry. SYBR-14, which likely requires membrane potential for… CONTINUE READING

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