Viability assessment of mammalian sperm using SYBR-14 and propidium iodide.

@article{Garner1995ViabilityAO,
  title={Viability assessment of mammalian sperm using SYBR-14 and propidium iodide.},
  author={D. Garner and L. Johnson},
  journal={Biology of reproduction},
  year={1995},
  volume={53 2},
  pages={
          276-84
        }
}
The proportion of living sperm in semen from six representative mammals was assessed by means of a dual staining technique using the stains SYBR-14 and propidium iodide (PI). SYBR-14, a newly developed fluorescent nucleic acid stain, maximally absorbs at 488 nm and emits at 518 nm when bound to DNA. Microscopic examination revealed that SYBR-14 stained the nuclei of living sperm bright green as determined by simultaneous examination of fluorescence and motility. Conversely, PI stained only… Expand
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