Viability and proliferation potential of adipose-derived stem cells following labeling with a positron-emitting radiotracer

@article{Elhami2011ViabilityAP,
  title={Viability and proliferation potential of adipose-derived stem cells following labeling with a positron-emitting radiotracer},
  author={Esmat Elhami and Andrew L. Goertzen and Bo Xiang and Jixian Deng and Chris Stillwell and Shadreck Mzengeza and Rakesh C Arora and Darren H. Freed and Ganghong Tian},
  journal={European Journal of Nuclear Medicine and Molecular Imaging},
  year={2011},
  volume={38},
  pages={1323-1334}
}
PurposeAdipose-derived stem cells (ASCs) have promising potential in regenerative medicine and cell therapy. Our objective is to examine the biological function of the labeled stem cells following labeling with a readily available positron emission tomography (PET) tracer, 18F-fluoro-2-deoxy-D-glucose (FDG). In this work we characterize labeling efficiency through assessment of FDG uptake and retention by the ASCs and the effect of FDG on cell viability, proliferation, transdifferentiation, and… 

18F-FDG Labeling of Mesenchymal Stem Cells and Multipotent Adult Progenitor Cells for PET Imaging: Effects on Ultrastructure and Differentiation Capacity

Direct labeling of MSCs and MAPCs with 18F-FDG is a suitable technique to noninvasively assess cell delivery and early retention with PET and confirmed that the main cellular properties are not rigorously affected.

PET Cell Tracking Using 18F-FLT is Not Limited by Local Reuptake of Free Radiotracer

A novel method of cell tracking using 3′-deoxy-3′-L-[18F]-fluorothymidine (18F-FLT) is assessed, which it is hypothesised will result in minimal local radiotracer reuptake and allow a more accurate estimation of cell retention.

Assessment of three techniques for delivering stem cells to the heart using PET and MR imaging

Direct injection of stem cells into the myocardium is the most effective way for cell transplantation to the heart in heart failure models and PET-MR imaging is a reliable technique for noninvasive tracking of implanted stem cells in vivo.

[89Zr]Oxinate4 for long-term in vivo cell tracking by positron emission tomography

[89Zr]Oxinate4 offers a potential solution to the emerging need for a long half-life PET tracer for cell tracking in vivo and deserves further evaluation of its effects on survival and behaviour of different cell types.

Molecular Imaging of Stem Cells and Exosomes for Myocardial Regeneration

The most reliable techniques for stem cell imaging in cardiac injury animal models, new and notable advancements in the field, and possible directions for cardiac regenerative medicine are outlined.

[ 89 Zr]Oxinate 4 for long-term in vivo cell tracking by positron emission tomography

89 Zr Oxinate 4 offers a potential solution to the emerging need for a long half-life PET tracer for cell tracking in vivo and deserves further evaluation of its effects on survival and behaviour of different cell types.

Molecular imaging for In vivo tracking of stem cell fate

Current imaging techniques for tracking stem cells are reviewed, with an emphasis on the advantages and drawbacks of each imaging approach, and future prospects for in vivo tracking of stem cells in regenerative medicine are discussed.

References

SHOWING 1-10 OF 25 REFERENCES

Ex vivo cell labeling with 64Cu–pyruvaldehyde-bis(N4-methylthiosemicarbazone) for imaging cell trafficking in mice with positron-emission tomography

Results indicate that in vivo imaging of cell trafficking is possible with 64Cu–PTSM-labeled cells and given the longer t1/2 of 64Cu relative to 18F, longer cell-tracking periods should be possible now with PET.

Fluorodeoxyglucose uptake in human cancer cell lines is increased by hypoxia.

Hypoxia increases cellular uptake of FDG in two different malignant human cell lines and increased glucose transport, in part due to increased membrane expression of the Glut-1 glucose transporter, contributes to this phenomenon.

Imaging of Cell Proliferation: Status and Prospects

The most promising use for thymidine and its analogs is in monitoring tumor treatment response, as demonstrated in animal studies and pilot human trials.

Fat tissue: an underappreciated source of stem cells for biotechnology.

Human adipose tissue is a source of multipotent stem cells.

To confirm whether adipose tissue contains stem cells, the PLA population and multiple clonal isolates were analyzed using several molecular and biochemical approaches and PLA cells exhibited unique characteristics distinct from those seen in MSCs, including differences in CD marker profile and gene expression.

In vivo validation of 3'deoxy-3'-[(18)F]fluorothymidine ([(18)F]FLT) as a proliferation imaging tracer in humans: correlation of [(18)F]FLT uptake by positron emission tomography with Ki-67 immunohistochemistry and flow cytometry in human lung tumors.

  • H. VesselleJ. Grierson D. Wood
  • Medicine
    Clinical cancer research : an official journal of the American Association for Cancer Research
  • 2002
FLT PET may be used to noninvasively assess proliferation rates of lung masses in vivo and play a significant role in the evaluation of indeterminate pulmonary lesions, in the prognostic assessment of resectable NSCLC, and possibly in the Evaluation of NSCLCs response to chemotherapy.

Systemic Delivery of Bone Marrow–Derived Mesenchymal Stem Cells to the Infarcted Myocardium: Feasibility, Cell Migration, and Body Distribution

Systemic intravenous delivery of BM-MSCs to rats after MI, although feasible, is limited by entrapment of the donor cells in the lungs, which enhances migration and colonization of the cells preferentially to the ischemic myocardium.

Imaging stem cells implanted in infarcted myocardium.