Viability and isolation of marine bacteria by dilution culture: theory, procedures, and initial results.

@article{Button1993ViabilityAI,
  title={Viability and isolation of marine bacteria by dilution culture: theory, procedures, and initial results.},
  author={Don K. Button and Frits Schut and Pham X. Quang and Rebeca Mart{\'i}n and Bronwyn R. Robertson},
  journal={Applied and environmental microbiology},
  year={1993},
  volume={59 3},
  pages={881-91}
}
Dilution culture, a method for growing the typical small bacteria from natural aquatic assemblages, has been developed. Each of 11 experimental trials of the technique was successful. Populations are measured, diluted to a small and known number of cells, inoculated into unamended sterilized seawater, and examined three times for the presence of 10 or more cells per ml over a 9-week interval. Mean viability for assemblage members is obtained from the frequency of growth, and many of the… CONTINUE READING
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