Viability and function in lymphocytes cultured from the horse, chicken, and mouse: effects of different leukocyte enrichment techniques.

@article{Mustafa2008ViabilityAF,
  title={Viability and function in lymphocytes cultured from the horse, chicken, and mouse: effects of different leukocyte enrichment techniques.},
  author={Amjad Mustafa and L Gillmeister and Wenndy Hernandez and Cathrine T Larsen and Sharon G. Witonsky and Steven David Holladay and Roy Patrick Kerr and Sattar Ansar Ahmed and Atsushi Santo and Robert M. Gogal},
  journal={Journal of immunoassay & immunochemistry},
  year={2008},
  volume={29 4},
  pages={
          370-89
        }
}
Methods of lymphocyte enrichment tend to vary across species, with the most common techniques employed being density-gradient separation and erythrocyte lysis buffer enrichment. In this study, we assessed lymphocyte viability and proliferation of avian, equine, and murine lymphocytes enriched by a commercial density-gradient technique and under identical, standardized culture conditions. The results of this study clearly show that, under identical enrichment and culture conditions, lymphocyte… CONTINUE READING
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