Viability and acrosome staining of bull, boar and rabbit spermatozoa.

  title={Viability and acrosome staining of bull, boar and rabbit spermatozoa.},
  author={Andras Kovacs and Robert Foote},
  journal={Biotechnic & histochemistry : official publication of the Biological Stain Commission},
  volume={67 3},
A practical and reliable staining procedure was developed to distinguish the viability and acrosomal status of bull, boar and rabbit spermatozoa. The first stain with trypan blue or Congo red is rapid and avoids artifacts. This stain is precipitated by neutral red during the 2 min required for fixation. The precipitate gives a high contrast black color, resistant to the subsequent rinsing and persists during the time required for staining the acrosome with Giemsa. Ten classes of spermatozoa are… CONTINUE READING

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