Vesicular proteins exocytosed and subsequently retrieved by compensatory endocytosis are nonidentical

@article{Wienisch2006VesicularPE,
  title={Vesicular proteins exocytosed and subsequently retrieved by compensatory endocytosis are nonidentical},
  author={Martin Wienisch and J{\"u}rgen Klingauf},
  journal={Nature Neuroscience},
  year={2006},
  volume={9},
  pages={1019-1027}
}
Upon exocytosis, synaptic vesicle proteins are released into the plasma membrane and have to be retrieved by compensatory endocytosis. When green fluorescent protein–labeled versions of the vesicle proteins synaptobrevin-2 and synaptotagmin-1 are overexpressed in rat hippocampal neurons, up to 30% are found on axonal membranes under resting conditions. To test whether and to what extent these plasma membrane–stranded proteins participate in exo-endocytic cycling, a new proteolytic approach was… 
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