Vesicular and non-vesicular sterol transport in living cells. The endocytic recycling compartment is a major sterol storage organelle.

@article{Hao2002VesicularAN,
  title={Vesicular and non-vesicular sterol transport in living cells. The endocytic recycling compartment is a major sterol storage organelle.},
  author={Mingming Hao and Sharron X. Lin and Ola J Karylowski and Daniel W{\"u}stner and Timothy E McGraw and Frederick R Maxfield},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 1},
  pages={609-17}
}
We examined the intracellular transport of sterol in living cells using a naturally fluorescent cholesterol analog, dehydroergosterol (DHE), which has been shown to mimic many of the properties of cholesterol. By using DHE loaded on methyl-beta-cyclodextrin, we followed this cholesterol analog in pulse-chase studies. At steady state, DHE co-localizes extensively with transferrin (Tf), a marker for the endocytic recycling compartment (ERC), and redistributes with Tf in cells with altered ERC… CONTINUE READING