Vesicle and vesicle-free extracellular proteome of Paracoccidioides brasiliensis: comparative analysis with other pathogenic fungi.

@article{Vallejo2012VesicleAV,
  title={Vesicle and vesicle-free extracellular proteome of Paracoccidioides brasiliensis: comparative analysis with other pathogenic fungi.},
  author={Milene Carmes Vallejo and Ernesto S. Nakayasu and Alisson Leonardo Matsuo and Tiago Jose Paschoal Sobreira and Larissa Valle Guilhen Longo and Luciane Ganiko and Igor C. Almeida and Rosana Puccia},
  journal={Journal of proteome research},
  year={2012},
  volume={11 3},
  pages={1676-85}
}
Microorganisms release effector molecules that modulate the host machinery enabling survival, replication, and dissemination of a pathogen. Here we characterized the extracellular proteome of Paracoccidioides brasiliensis at its pathogenic yeast phase. Cell-free culture supernatants from the Pb18 isolate, cultivated in defined medium, were separated into vesicle and vesicle-free fractions, digested with trypsin, and analyzed by liquid chromatography-tandem mass spectrometry. In vesicle and… CONTINUE READING