Very high expression of an anti-carcinoembryonic antigen single chain Fv antibody fragment in the yeast Pichia pastoris.

@article{Freyre2000VeryHE,
  title={Very high expression of an anti-carcinoembryonic antigen single chain Fv antibody fragment in the yeast Pichia pastoris.},
  author={Fabio Freyre and Jos{\'e} Eduardo Rivo V{\'a}zquez and Marta Ayala and Leonardo Cana{\'a}n-Haden and Howard Bell and Isabel Rodr{\'i}guez and Antonio L{\'o}pez Gonz{\'a}lez and Alberto Cintado and Jorge V. Gavilondo},
  journal={Journal of biotechnology},
  year={2000},
  volume={76 2-3},
  pages={157-63}
}
In this paper we report the development of a recombinant strain of the yeast Pichia pastoris, which secretes an anti-carcinoembryonic antigen single chain Fv (scFv) antibody fragment to the culture supernatant as a biologically active protein, at levels of 1.2 g l(-1). The yeast scFv was purified by IMAC, with a final yield of approximately 0.440 g of 93% pure scFv per liter of culture supernatant. The specific activity in ELISA of the yeast scFv was almost three times higher than that of a… CONTINUE READING

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