Vertical agarose gel electrophoresis and electroblotting of high-molecular-weight proteins.

  title={Vertical agarose gel electrophoresis and electroblotting of high-molecular-weight proteins.},
  author={Chad M. Warren and Paul R. Krzesinski and M. L. Greaser},
  volume={24 11},
The electrophoretic separation of high-molecular-weight proteins (> 500 kDa) using polyacrylamide is difficult because gels with a large enough pore size for adequate protein mobility are mechanically unstable. A 1% vertical sodium dodecyl sulfate (SDS)-agarose gel electrophoresis (VAGE) system has been developed that allows titin (a protein with the largest known SDS subunit size of 3000-4000 kDa) to migrate over 10 cm in a approximately 13 cm resolving gel. Such migration gives clear and… CONTINUE READING
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