Vertical agarose gel electrophoresis and electroblotting of high-molecular-weight proteins.

@article{Warren2003VerticalAG,
  title={Vertical agarose gel electrophoresis and electroblotting of high-molecular-weight proteins.},
  author={Chad M. Warren and Paul R. Krzesinski and M. L. Greaser},
  journal={Electrophoresis},
  year={2003},
  volume={24 11},
  pages={1695-702}
}
The electrophoretic separation of high-molecular-weight proteins (> 500 kDa) using polyacrylamide is difficult because gels with a large enough pore size for adequate protein mobility are mechanically unstable. A 1% vertical sodium dodecyl sulfate (SDS)-agarose gel electrophoresis (VAGE) system has been developed that allows titin (a protein with the largest known SDS subunit size of 3000-4000 kDa) to migrate over 10 cm in a approximately 13 cm resolving gel. Such migration gives clear and… CONTINUE READING
Highly Influential
This paper has highly influenced 14 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS
Recent Discussions
This paper has been referenced on Twitter 2 times over the past 90 days. VIEW TWEETS
110 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 110 extracted citations

Similar Papers

Loading similar papers…