Vertebral landmarks for the identification of spinal cord segments in the mouse

@article{Harrison2013VertebralLF,
  title={Vertebral landmarks for the identification of spinal cord segments in the mouse},
  author={Megan Harrison and Aine O'Brien and L Adams and Gary J. Cowin and Marc J. Ruitenberg and Gulgun Sengul and Charles Watson},
  journal={NeuroImage},
  year={2013},
  volume={68},
  pages={22-29}
}
Accurate identification of spinal cord segments in relation to vertebral landmarks is essential to surgery aimed at experimental spinal cord injury. [...] Key Method We have analyzed a complete series of high-resolution magnetic resonance (MR) images from the mouse spine in order to delineate the boundaries of spinal cord segments in relation to vertebral landmarks. The resulting atlas can be used to plan experimental approaches that require the accurate identification of a target spinal cord segment.Expand
Prediction Algorithm of the Cat Spinal Segments Lengths and Positions in Relation to the Vertebrae
TLDR
An algorithm to predict spinal segments locations based on their relation to vertebral reference points is developed and can be further translated to other species including human. Expand
The Mouse Spinal Cord (Medulla Spinalis)
The mouse spinal cord is the caudal most part of the central nervous system (CNS) and that one which in adult life still allows to recognize the origin from the cylindrical neural tube. The spinalExpand
Sacral Spinal Cord Transection and Isolated Sacral Cord Preparation to Study Chronic Spinal Cord Injury in Adult Mice
TLDR
A mouse model of chronic spinal cord injury is generated that reliably reproduces spasticity observed after SCI, while at the same time allows study of motor impairments in vivo and in an in vitro preparation of the spinal cord. Expand
Tools for efficient analysis of neurons in a 3D reference atlas of whole mouse spinal cord
TLDR
These tools provide high-throughput analyses of whole mouse SC and enable direct comparison of 3D spatial information between animals and studies and verified mapping accuracies for known neurons and demonstrated the usefulness of this platform to reveal unknown neuronal distributions. Expand
Imaging spinal cord activity in behaving animals
TLDR
The recent development of imaging methods for real-time interrogation of cellular activity in the spinal cord of behaving mice has removed longstanding technical obstacles to spinal cord research and allowed new insight into how different cell types encode sensory information from mechanoreceptors and nociceptors in the skin. Expand
Rostral lumbar segments are the key controllers of hindlimb locomotor rhythmicity in the adult spinal rat.
TLDR
Results demonstrate in adult rats that rostral lumbar segments are essential for the regulation of hindlimb locomotor rhythmicity and that the more caudal spinal networks alone cannot control locomotion in the absence of the rostrals around L2 even when loadbearing rhythmic proprioceptive afferent input is imposed. Expand
Imaging in vivo dynamics of sensory axon responses to CNS injury
TLDR
The dynamic changes that injured sensory axons undergo and methods of imaging them in vivo are described and the possibility of in vivo imaging of subcellular dynamics within the axon is described. Expand
Correlation of in vivo and ex vivo 1H-MRI with histology in two severities of mouse spinal cord injury
TLDR
In vivo MRI has the potential to accurately identify in mice the progression of tissue alterations induced by SCI and is successfully implemented by ex vivo MRI examination, and provides a valuable approach for further studies intended to evaluate therapeutic strategies on SCI. Expand
Induction of Complete Transection-Type Spinal Cord Injury in Mice.
TLDR
A surgical protocol for inducing a complete transection type injury in C57BL/6 mice at thoracic vertebral level 10 (T10) is developed, which leads to reproducible transections-type injury in small rodents with minimal collateral muscle and bone damage and therefore minimizes confounding factors. Expand
Synchrotron radiation microtomography of brain hemisphere and spinal cord of a mouse model of multiple sclerosis revealed a correlation between capillary dilation and clinical score
TLDR
A three‐dimensional structural analysis of brain and spinal cord tissues of a mouse model of multiple sclerosis, known as experimental autoimmune encephalomyelitis (EAE), suggests that vasodilation and vacuolation are related with neurological symptoms ofmultiple sclerosis. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 38 REFERENCES
Atlas of the Mouse Spinal Cord
This chapter provides the fundamental structural map of the spinal cord of the principal laboratory research animal, the mouse, and describes issues and procedures that are used in preparation ofExpand
Quantitative MRI of spinal cord injury in a rat model
TLDR
These studies suggest that it is possible to use MRI to detect the onset of secondary injury in the spinal cord and indicate that early detection of MRI‐visible pathology may provide the necessary markers for predicting the long‐term level of neurologic deficit. Expand
The Organization of the Spinal Cord
This chapter introduces the general anatomy and organization of the spinal cord. The spinal cord is the part of the central nervous system that controls the voluntary muscles of the limbs and trunk,Expand
A method for in vivo high resolution MRI of rat spinal cord injury
TLDR
An implanted radiofrequency coil is developed to obtain high resolution in vivo MR images at 1.9 Tesla of rat spinal cords that have been injured using a standardized weight drop technique, which afford the possibility for longitudinal studies of experimental spinal cord injury before and after treatment. Expand
Recovery from spinal cord injury: A new transection model in the C57Bl/6 mouse
TLDR
It is shown that functional recovery from complete transection of the adult mouse spinal cord can in fact occur without any intervention if dural injury along with displacement of the ends of the cut cord and fibroblastic infiltration is minimized. Expand
In vivo magnetic resonance imaging of spinal cord injury in the mouse.
TLDR
MRI was used to detect and monitor iron-labeled mesenchymal stem cells after their transplantation into the injured cord and MSCs appeared as large, obvious regions of signal loss in the cord, which decreased in size over time. Expand
Spinal cord
TLDR
The rostrocaudal organization of the spinal cord is associated with regional innervation patterns and its operation includes the integrative processing of cutaneous, musculoskeletal and autonomic function. Expand
In vivo high‐resolution imaging of the injured rat spinal cord using a 3.0T clinical MR scanner
To investigate the feasibility of obtaining high‐resolution MR images for the detection of pathological changes occurring in the injured rat spinal cord with a routine clinical 3.0T imaging system.
Traumatic spinal cord injury produced by controlled contusion in mouse.
TLDR
The results demonstrate that the electromagnetic spinal cord injury device (ESCID) can be used to produce a well-controlled contusion injury in mice and proves the shape of the force recording proved to be a highly sensitive measure of subtle variations in the spinal compartment that were otherwise difficult to detect in this small species. Expand
Cytoarchitecture of the Spinal Cord
TLDR
The chapter discusses the major cytological characteristics of each of the laminae, which have shown that neurons within a lamina often show distinct dendritic architecture, chemoarchitecture and patterns of connections as well as function. Expand
...
1
2
3
4
...