Versatility of using major histocompatibility complex class II dextramers for derivation and characterization of antigen-specific, autoreactive T cell hybridomas.

@article{Krishnan2015VersatilityOU,
  title={Versatility of using major histocompatibility complex class II dextramers for derivation and characterization of antigen-specific, autoreactive T cell hybridomas.},
  author={Bharathi N. Krishnan and Chandirasegaran Massilamany and Rakesh H. Basavalingappa and Rajkumar A. Rajasekaran and Charles A. Kuszynski and Barbara L Switzer and Daniel A. Peterson and Jay Reddy},
  journal={Journal of immunological methods},
  year={2015},
  volume={426},
  pages={86-94}
}
Antigen-specific, T cell hybridomas are useful to study the cellular, molecular and functional events, but their generation is a lengthy process. Thus, there is a need to develop robust methods to generate the hybridoma clones rapidly in a short period of time. To this end, we have demonstrated a novel approach using major histocompatibility complex (MHC) class II dextramers to generate T cell hybridomas for an autoantigen, proteolipid protein (PLP) 139-151. Using MHC class II dextramers… CONTINUE READING