Versatile transformation vectors to assay the promoter activity of DNA elements in plants.

@article{Pasquali1994VersatileTV,
  title={Versatile transformation vectors to assay the promoter activity of DNA elements in plants.},
  author={Gemma Pasquali and Pieter B F Ouwerkerk and Johan Memelink},
  journal={Gene},
  year={1994},
  volume={149 2},
  pages={373-4}
}
A convenient vector system was developed to evaluate transcriptional promoter activities in plants. Two primary vectors, optionally containing the cauliflower mosaic virus (CaMV) 35S -47 or -90 minimal promoters, offer multiple sites for cloning the sequence of interest upstream from the beta-glucuronidase gene (gusA). The promoter-gusA cassette can be transferred to a binary vector containing the selectable neomycin phosphotransferase II-encoding gene (nptII) next to the left border. In… CONTINUE READING

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