Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria.

@article{Quandt1993VersatileSV,
  title={Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria.},
  author={J{\"u}rgen Quandt and Michael F. Hynes},
  journal={Gene},
  year={1993},
  volume={127 1},
  pages={15-21}
}
A set of vector plasmids which greatly facilitate gene replacement and reverse genetics in many Gram-negative bacteria was constructed. These vectors are based on the P15A origin of replication (ori) and incorporate sacB from Bacillus subtilis, which is inducible by sucrose and is lethal when expressed in Gram-negative bacteria. The vectors also have a convenient antibiotic-resistance marker (gentamicin resistance) and the lacZ alpha system which allows blue/white selection of cloned fragments… CONTINUE READING
Highly Influential
This paper has highly influenced 31 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 430 extracted citations

Similar Papers

Loading similar papers…