Versatile multi-functionalization of protein nanofibrils for biosensor applications.

@article{Sasso2014VersatileMO,
  title={Versatile multi-functionalization of protein nanofibrils for biosensor applications.},
  author={Luigi Sasso and Sandy Suei and Laura J. Domigan and Jackie P. Healy and Volker Nock and M. A. K. Williams and Juliet A Gerrard},
  journal={Nanoscale},
  year={2014},
  volume={6 3},
  pages={
          1629-34
        }
}
Protein nanofibrils offer advantages over other nanostructures due to the ease in their self-assembly and the versatility of surface chemistry available. Yet, an efficient and general methodology for their post-assembly functionalization remains a significant challenge. We introduce a generic approach, based on biotinylation and thiolation, for the multi-functionalization of protein nanofibrils self-assembled from whey proteins. Biochemical characterization shows the effects of the… 
Crystallin Nanofibrils: A Functionalizable Nanoscaffold with Broad Applications Manufactured from Waste.
TLDR
In this study, a successful method for the functionalization of crystallin protein nanofibrils by immobilizing several enzymes of industrial relevance through a glutaraldehyde cross-linking approach is reported.
Polyethyleneimine-modified graphene oxide nanocomposites for effective protein functionalization.
TLDR
Data suggest that the GO@BPEI composites will have great potential as scaffolds for proteins fully exerting their biofunctions, which are superior to other immobilized materials.
Protein Fibril-Templated Biomimetic Synthesis of Highly Fluorescent Gold Nanoclusters and Their Applications in Cysteine Sensing
TLDR
Self-assembled bovine serum albumin (BSA) amyloid-like nanofibers are used as the bioinspired scaffold for the synthesis of Au NCs and the use of Fib-Au NCs for cysteine (Cys) sensing both in vitro and inside live cells is demonstrated.
Amyloid-directed assembly of nanostructures and functional devices for bionanoelectronics.
Amyloids have the potential to serve as useful building blocks for functional materials and bionanoelectronic devices because they possess precisely assembled fibrillar structures, tunable
Hybrid Nanoscopy of Hybrid Nanomaterials.
TLDR
This study represents an important step forward in the characterization of multifunctionalized hybrid materials, a key challenge in nanoscience.
Protein microgels from amyloid fibril networks.
TLDR
It is shown that protein nanofibrils can be used to form the basis of monodisperse microgels and gel shells composed of naturally occurring proteins, and that these materials are nontoxic to human cells and that they can be use to enhance the efficacy of antibiotics relative to delivery in homogeneous solution.
Protein Microgels from Amyloid Fibril Networks.
TLDR
The propensity of proteins to self-assemble into amyloid fibrils can be exploited under microconfinement, afforded by droplet microfluidic techniques, and this approach allows the generation of multi-scale functional microgels that can host biological additives and can be designed to incorporate additional functionality, such as to aid targeted drug delivery.
Amyloid fibril based bionanotechnologies.
TLDR
Electrochemistry studies demonstrated that functionalised PNFs can be successfully used to develop single enzyme-, or dual enzyme-based biosensing elements, for glucose and lactose analysis, and surface-assembly studies established that PNF scaffold provides more surface area for biomolecule immobilisation, and can be used to create PNF-based active surfaces.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 60 REFERENCES
Amyloid fibrils as a nanoscaffold for enzyme immobilization
TLDR
The incorporation of the GOD‐functionalized amyloid fibrils into PVOH provides an excellent ‘proof of concept’ model for the creation of a new bionanomaterial using a functionalizedAmyloid Fibril scaffold.
Characterization of peptide-nanostructure-modified electrodes and their application for ultrasensitive environmental monitoring.
TLDR
The organized incorporation of novel biocompatible organic nanostructures into extremely sensitive amperometric biosensors is demonstrated and the high sensitivity reported may provide a novel platform for biosensor design and fabrication for environmental monitoring, homeland security, and other applications.
Multifunctional Nanorods for Biomedical Applications
TLDR
Functionalized multicomponent nanorods are utilized in applications ranging from multiplexing, protein sensing, glucose sensing, imaging, biomolecule-associated nanocircuits, gene delivery and vaccinations.
Multiple Self‐Assembly Functional Structures Based on Versatile Binding Sites of β‐Lactoglobulin
In recent years, research in the field of protein-based fibrils gained a great attention due to use of these materials as building blocks for construction of functional synthetic biofilms. Yet,
Peptide nanotubes: molecular organisations, self-assembly mechanisms and applications
TLDR
The present review addresses the recent advances in their fundamental comprehension and mechanistic aspects of their latest downstream uses, including the Lanreotide peptide monodisperse nanotubes, as well as the kinetic and thermodynamic aspects of the corresponding self-assembly processes.
...
1
2
3
4
5
...