Versatile Recombinant SUMOylation System for the Production of SUMO-Modified Protein

@inproceedings{Weber2014VersatileRS,
  title={Versatile Recombinant SUMOylation System for the Production of SUMO-Modified Protein},
  author={Alain R. Weber and David Schuermann and Primo Sch{\"a}r},
  booktitle={PloS one},
  year={2014}
}
Posttranslational modification by small ubiquitin-like modifiers (SUMO) is being associated with a growing number of regulatory functions in diverse cellular processes. The biochemical investigation into the underlying molecular mechanisms, however, has been lagging behind due to the difficulty to generate sufficient amounts of recombinant SUMOylated proteins. Here, we present two newly designed two-component vector systems for the expression and purification of SUMO-modified target proteins in… CONTINUE READING
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