Venom Collection from Honey Bees

@article{Benton1963VenomCF,
  title={Venom Collection from Honey Bees},
  author={Allen W. Benton and Roger A. Morse and Joseph Dickson Stewart},
  journal={Science},
  year={1963},
  volume={142},
  pages={228 - 230}
}
A device that provides an electric shock makes it possible to collect pure venom from several thousand honey bees (Apis mellifera). The collection apparatus fits underneath the brood chamber of a colony of bees and may be moved from hive to hive. Each colony is "milked" for 5 minutes. An average of 20 hives must be "milked" to obtain 1 gram of venom. Under optimum conditions this quantity of venom is produced by 10,000 worker bees. 
Notes on Venom Collection from Honeybees
TLDR
The method of venom collection referred to here was designed for bulk collection, and yields a gram of dried venom in five minutes' ‘milking’ of each, of 20 hives, similar to the delicate method described by D. J. Palmer in Bee World in 1961. Expand
An Improved Method for Collecting the Liquid Fraction of Bee Venom
SUMMARYBy introducing a cooling system into an existing apparatus for collecting bee venom, it was possible to obtain large enough quantities of the liquid fraction to facilitate chemical analysis.Expand
Standard methods for Apis mellifera venom research
Honey bees have a sting which allows them to inject venomous substances into the body of an opponent or attacker. As the sting originates from a modified ovipositor, it only occurs in the femaleExpand
Adaptation of the electrical stimulation procedure for the collection of vespid venoms.
TLDR
Venom was obtained from yellow hornets, bald-faced hornets and yellow jacket species by modification of the electrical “milking” method, a substantial improvement over previous techniques employed to obtain pure vespid venoms. Expand
A qualitative analysis of the proteins in the venom of honey bees.
TLDR
A comparison of the proteins in whole insect extracts and those found in the pure venom indicates an entirely different protein pattern with virtually no cross-matching. Expand
Venoms of Apidae
TLDR
The venom injected by the stinging honey bee has been the subject of some of the most exhaustive and extensive research activities in the entire field of insect biochemistry. Expand
3 – Methods for the Collection of Venoms
TLDR
A prerequisite to studies on the nature of the venoms was the development of methods for their collection, before collection of venom could be possible, the Hymenoptera must be collected in the field or reared in or near the laboratory. Expand
Measurements of Stinging Behaviour in Individual Worker Honeybees (Apis Mellifera L.)
TLDR
The procedure described allows natural stinging behaviour to be elicited in the laboratory without the possibility of group effects and free from the complicated sets of environmental conditions that are unavoidable when entire colonies are tested in an apiary. Expand
The Efficacy of a New Modified Apparatus for Collecting Bee Venom in Relation to Some Biological Aspects of Honeybee Colonies
TLDR
Results indicated that the modified device of gathering bee venom from hives was successfully gave adequate quantities of bee venom along the period of the experiment, March 2012 to November 2012, and the best period for collecting bee venom was between 4 pm to 6 pm at August month. Expand
Venom Collection from Species of Honeybees in South-East Asia
TLDR
The first expedition to collect samples of venom from the tropical species of Apis mellifera is described, which is likely to be the first expedition of its kind. Expand
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References

SHOWING 1-8 OF 8 REFERENCES
Bioassay and Standardization of Venom of the Honey Bee
TLDR
A reverse phase, bioassay method of standardizing solutions of Hymenoptera venom is proposed and a unit of venom is defined. Expand
Hymenoptera: Pure Venom from Bees, Wasps, and Hornets
Pure venom can be obtained from bees, wasps, and hornets by electrical stimulation with inexpensive apparatus.
Antigenic relationships between honeybees, wasps, yellow hornets, black hornets, and yellow jackets
TLDR
Gel diffusion studies indicate that yellow jacket, yellow hornet, black horne, wasp, and honeybee contain common antigens, and that a satisfactory antigen mixture should include at least bee, wasP, yellow jackets, and hornet extracts. Expand
Protease Inhibitor from Groundnut Skins
TLDR
The present state of the research of the hæmostatic factor from peanuts (Arachis hypogaea, L.) (groundnuts) was recently reviewed in Nature by Astrup, Brakman and Sjolin and an active protease inhibitor was isolated. Expand
The teaching of preventive medicine in the United States
TLDR
In the teaching of public health in Great Britain the authors have much to learn from the schools in the United States, particularly for health officers in active practice who wish to concentrate their attention on special subjects. Expand