Vectors with hidden cloning sites.

@article{Welker2000VectorsWH,
  title={Vectors with hidden cloning sites.},
  author={Ervin Welker and Andr{\'a}s V{\'a}radi},
  journal={Biochemical and biophysical research communications},
  year={2000},
  volume={271 2},
  pages={
          534-6
        }
}
A general strategy is described for using the cleavage site of restriction enzymes in vectors for cloning regardless of how many sites the given enzymes have in the vector. The application of this method allows one to open any vector at its cloning site with protruding ends which can be compatible with almost every commercially available Class II restriction enzyme. By employing this method, the laborious construction of new vectors can be simplified considerably. This general strategy is based… CONTINUE READING
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